Do you know of any software out there that I can use to measure reign of interest of tiff files for a scratch assay experiment?

currently I am using Fiji’s freehand selection and measuring area around the cell cluster at two different time point (usually beginning and end) to determine the extant of invasion . However, this process is very time consuming; especially when you have like 288 locations . There must be something out there that can helps with automate, any suggestion or feedback would greatly be appreciated

Hi @red.grean, I was reading another discussion and thought about your problem. I was wondering if the stardist method might also work for your data?

Discussion below:

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